Mobile learning application developers have to overcome inherent limitations imposed by mobile devices in order to produce usable applications. There are usability guidelines available to assist in the design process but with technological improvements, these guidelines should be updated and extended. In this research, we proposed an updated usability guideline to assist in the design process of mobile learning applications. A systematic analysis of seventeen usability studies on mobile learning application was conducted, listed usability problems were extracted and analyzed to formulate new usability guidelines and subsequently the guidelines were updated. The validation results indicate that the updated guideline helps to achieve a higher level of usability as compared to previous guidelines.