Internationally, there has been considerable literature on the predictors of academic performance in higher education. However, the empirical findings are still limited in Cambodia; therefore, this paper was conducted to examine the determinants of academic performance of 329 undergraduate students selected from a university in Cambodia. Based on the findings from the multivariate regression analysis, a number of variables, namely high school grade, English ability, class attendance, study effort, academic self-efficacy, and family socio-economic status were found to be positively associated with academic performance. Term-time employment and family size had an adverse impact on academic performance. However, there was no sufficient evidence to indicate age, gender, household location, parental education, parental involvement, and teaching evaluation to have predictive power on academic performance. Hence, the study concluded that the personal backgrounds played the potential role in predicting academic performance of undergraduate students.