The low achievement of chemistry students in Nigerian Colleges of Education has been attributed to a number of factors including a lack of indigenous computer software for enhancing course teaching. This study therefore sought to develop and validate an instructional computer package on organic chemistry for Colleges of Education in Nigeria. The development of the package involved the use of Macromedia Flash and Macromedia Time Line and Frames on HTML platform. CorelDraw and Microsoft Word programs were used for texts and graphics, Macromedia Fireworks was used for some texts, buttons, and graphics, while Macromedia Flash was utilised for animations. In the end, an organic chemistry computer instructional package (OCIP) has been produced and validated. It is recommended among others that lecturers teaching organic chemistry to first year College of Education students should make use of the developed package to improve the understanding of the course by the students.