Universities use prerequisites to regulate the path of students through a program or major. However, the impact of prerequisites on follow-up courses in anatomy and physiology is not well understood. If success in follow-up courses depends on completion of prerequisite courses, then students should earn higher grades on exam questions that assess prerequisite knowledge. To test this hypothesis, we investigated the potential impact of a required prerequisite human physiology lecture course on a followup human physiology laboratory course and a follow-up molecular pharmacology course. We also investigated the potential impact of a recommended prerequisite human physiology laboratory course on a follow-up human anatomy course. We assessed student exam performance in the follow-up courses based on their familiarity with the material from the required prerequisite course or the recommended prerequisite course. Our results were mixed and demonstrated limited performance gains in the follow-up courses despite overlap of material among the courses. These results suggest that prerequisite courses may not have a significant impact on the outcome of future related courses and that individual academic programs should evaluate the effectiveness of prerequisite courses in a local context.