Distance education particularly the IT based distance learning is a fast growing concept in the academic world. The employees working in such distance learning institutions have certain issues that need to be investigated. One important such issue the job satisfaction of employees. Job satisfaction of employees can be investigated through several variables. Job satisfaction is equally important in all the organizations including the face to face and distance learning organizations. Distance learning organizations differ from face to face as the supervisor and the employees happen to be seated hundreds of miles away from each other. They do not even have a facial know how or interaction with each other. This distance create certain differences in the culture and the facial anonymity also has an impact on the supervisor support and other issues as well. This study intended to analyze the impact of organizational culture, organizational communication and supervisor support on the job satisfaction of the employees working in the IT based distance learning institutions of Pakistan. These variables are so selected because the employees working in such organizations happen to be seated away from supervisor and one another as well. A survey was conducted for this purpose in which data was collected through online questionnaire and data processed through regression analysis. Findings of the study revealed that all the three variables including organizational culture, organizational communication and supervisor support has a positive impact on the job satisfaction of employees working in the IT based distance learning organizations.