The present study aimed at comparing the level of cultural intelligence among teachers and university students, and to define whether there are statistically significant differences in the level of cultural intelligence due to gender variables. The sample consisted of 300 teachers and 400 students at the Hashemite University, chosen by random selection, and data collected using the Cultural Intelligence Scale. Results of the study showed a high level of cultural intelligence among teachers and university students and also showed Statistically significant differences were also found in the levels of both cognitive and behavioral cultural intelligence attributed to teachers and university students, in favor of teachers. And statistically significant differences in the level of motivational cultural intelligence in favor of male teachers, and statistically significant differences in the level of behavioral cultural intelligence in favor of female teachers. The results of the study also showed statistically significant differences in the levels of cultural intelligence, meta-cognitive cultural intelligence, cognitive cultural intelligence and motivational cultural intelligence in favor of male students.