The objective of this study is to produce a vignette in measuring mathematical knowledge for teaching based conceptual (MKT-C). The method of this research is a development research conducted by synthesizing the expert reviews on MKT-C in the knowledge domain of material content which was divided into sub domains: common content knowledge based conceptual (CCK-C), specialized content knowledge based conceptual (SCK-C) and knowledge at the mathematical horizon based conceptual (KMH-C) through vignette. The research procedure is as follows: defining constructs, identifying and preparing indicators, developing vignette, validating experts or expert validation and trial test. The research phases included (1) preliminary phase: preparation and design and (2) formative evaluation phase: self-evaluation, prototyping and field test (Tessmer, 2013). At formative evaluation phase, self-evaluation began and it is done by the researcher himself. In expert review phase, validation in terms of content, construct and language by experts was done. One-to-one phase was conducted for the teacher to test the readability. Then it was tried out to the teachers in the small group of non-research subjects. They were asked for suggestions and comments for improvement. The small group carried out was to 29 teachers who participated in the teacher professional education program at Universitas Halu Oleo.