This study aims to obtain information regarding the effect of the RPO on students' scientific literacy using global scientific literacy questionnaire (GSLQ) in terms of GSLQ dimension and gender differences. The quantitative research approach is used with experimental methods. The U Mann Whitney, Wilcoxon, T-Test, and descriptive statistics were used to determine whether there were differences in students' global scientific literacy between the control group and the experiment group. The results show that there are no significant differences in students' global scientific literacy between the control and experiment groups, nevertheless, the results in the experiment group show that RPO appears to have significant effects, especially in the habits of mind dimension and across students' gender. Male students in the experiment group are able to show better results on habits of mind dimension than female students. However, female students in the experiment group are able to show a higher increase than the students in the control group.