This study investigate to test and analyse the influence of organizational stressors include role ambiguity, role conflict, and workload on job performance among academic staff. The data were collected by structured questionnaires through cluster sampling techniques and 209 academic staff from research universities inside Malaysia completed the questionnaires. Partial least squares structural equation modeling (PLS-SEM), has been employed to perform the analysis. The outcomes of Smart PLS have been assessed through measurement model and structural model. The result identify that role ambiguity is the major predictors of organizational stressors that influenced adversely on academics performance. Also, role conflict is the second predictors that negatively effects on the job performance of academics staff but not workload. This research makes significant theoretical and practical implications to enrich the existing literature on organizational stressors and contribute to academic staff job performance, and help existing policy makers and administrators of universities to reduce adverse impacts of organizational stress factors and manage to enhance academics performance.