This study analyzes the use of e-portfolios in Higher Education, specifically, participants' opinion concerning various aspects of the experience and the teaching methods used. Its main purpose is to determine the influence of professors in student satisfaction in this virtual environment, through a descriptive study performed using a mixed focus that combines quantitative and qualitative data analysis. The data collection instruments were a questionnaire for recording the students' opinions and a structured interview of three professors. The study's sample of 251 was drawn from students who were enrolled in different subjects. According to the results obtained, the professor is a decisive variable in student satisfaction when analyzing pedagogical issues concerning the e-portfolio, students' opinions about the professor's teaching actions, and usability of the platform. The students' opinions differ most on issues of time management and collaborative learning, but also on issues such as participation, motivation, and reflection on learning.