Based on an experiment with 5452 kindergarten school children from 15 cities, this article examines the effect of a customised spiritual education programme (SEP) on their quality of life. Results showed that treatment group kindergarteners who participated in the SEP had higher scores on the Pictured Child Quality of Life Self-Questionnaire in comparison to the control group. Kindergarteners from European cities, US, Canadian and Australian cities, boys, Christian children, those who had done three to four rounds of the SEP during the study period and those who self-practiced the lessons, were more likely to have higher post-test scores on the quality of life scale. Based on the study results, a broad frame for having SEP for kindergarten school children across cultures has been suggested comprising the spiritual domains of calmness, silence and quiet; listening; accepting; loving nature; and, using imagination, resilience and efficiency in everyday functioning.