The aim of the study was describing the development and validation of students' perception on learning by questioning (LBQ) scale in physics. Research and Development (R & D) design was utilized in the research method. The number of senior high school students involved in the research were 224 students, composed of 95 boys (42.41%) and 129 girls (57.59%) from Indonesia. The instrument used for this study was the questionnaire of students' perception on LBQ. Analysis of data used SPSS 21 and AMOS 21, which consisted of identifying the factor structure through an exploratory factor analysis (EFA), estimating each dimension's internal consistency reliability coefficients, and cross-validating through a confirmatory factor analysis (CFA). The research obtained findings: (a) the construct validities and reliabilities of the students' perception on LBQ was varying from 0.577 and 0.901 with reliability Cronbach's [alpha] was 0.74 and the total variance explained was 57.99%, (b) the result of CFA determined the parameters such as RMSEA, GFI, AGFI, RMR, CFI, NFI, and IFI of the structural model of students' perception on LBQ, which may be considered an acceptable model.