The substantive purpose of this study was to identify the students' preferences to use asynchronous and synchronous e-learning resources. Asynchronous means that there is no set time for the learning to be occurring. Learners can learn anywhere and can consume their time to gain knowledge of what they want to know and when they need to know. Synchronous e-learning is related to structure and time bounded activities, which are offered through web conferencing and chatting options. E-learning resources give option to do one, or both. Population of the study comprised all the students of two public universities situated in Lahore. Subjects of the study comprised purposively selected 128 Male and 158 Female students. Data were collected only from those students who were having experience of synchronous along with asynchronous e-learning activities. A self-developed instrument was used to collect data. To validate the instrument a pilot study was done, which showed statistically significant reliability index (Cronbach Alpha) = 0.83. SPSS was used for data analysis. Independent sample t-test was used to find out difference in male and female students' opinions. Descriptive statistics were used to present students' responses related to different aspects of e-learning. Findings revealed statistically significant difference in students' responses regarding effectiveness of synchronous and asynchronous e-learning activities. Male students preferred synchronous as well as asynchronous e-learning activities more than female students at higher education level. Students' were found to have greater interest in synchronous activities when they had credit in terms of marks.