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ERIC Number: EJ1207878
Record Type: Journal
Publication Date: 2019
Pages: 5
Abstractor: As Provided
ISSN: ISSN-1470-8175
Exploring Ligand--Protein Interaction: A Laboratory Exercise on Herbicide Binding to Plasma Transport Protein
Roslan, Amira Adlin; Tayyab, Saad
Biochemistry and Molecular Biology Education, v47 n2 p156-160 Mar-Apr 2019
A laboratory exercise on the interaction between the herbicide pendimethalin (PM) and goat serum albumin (GSA), a carrier protein present in mammalian blood circulation, is described. Fluorescence spectroscopy was used to study the binding reaction between PM and GSA. Titration of a constant amount of the protein (GSA) with increasing ligand (PM) concentrations produced a consecutive decrease in the protein's fluorescence. Treatment of the fluorescence quenching data according to the Stern--Volmer equation yielded the values of the Stern--Volmer constant (K[subscript sv]) and bimolecular quenching rate constant (k[subscript q]), whereas values of the binding constant (K[subscript a]) and number of binding sites (n) were obtained from the double logarithmic plot. This experiment provides an exciting opportunity for undergraduate students to independently perform ligand binding studies with a protein, in addition to providing the means for the determination of their binding parameters.
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Publication Type: Journal Articles; Reports - Research
Education Level: Higher Education; Postsecondary Education
Audience: N/A
Language: English
Sponsor: N/A
Authoring Institution: N/A
Grant or Contract Numbers: N/A