The present study aimed to fill a gap in the literature by documenting the EAP needs of the research assistants of Nursing in a public Turkish university. The study was of a qualitative nature and data was collected by means of an open-ended questionnaire, whose questions were based on Hutchinson & Waters's (1987) framework to identify the target needs of language learners. The participants of the study were 8 research assistants working for the Nursing department of a public university in Turkey, who volunteered to participate in the study. For the analysis of data, the statements of the participants were coded according to their themes and topics. The findings of the study revealed that the participants were in need of improving their academic speaking and writing skills within the context of Nursing in order to perform better in writing research articles and giving presentations in international conferences.