Purpose: The study aimed to determine the validity of the Fitbit Charge 2 (FC2) for measuring PA steps in college physical education settings using the Yamax SW-200 (YX) as the criterion device. Methods: Undergraduate students (n = 17) worn both FC2 and YX in two conditions (i.e., controlled and college physical education settings). The absolute value of average percent error (APE) was calculated to evaluate the agreement between FC2 and YX in both conditions. Results: FC2 estimates of total steps in walking, jogging, and running in the controlled condition were within acceptable range (i.e., APE < 5%) without gender difference. However, FC2 tended to overestimate total steps per class in physical education teaching football and baseball units, and gender difference was significant while no difference in sport units were shown. Conclusions: FC2 revealed promising validity evidence measuring total steps in a controlled setting and its validity may be questionable in college physical education settings where student physical activity patters are irregular.