The number of students with disabilities attending universities is increased, and several challenges face them in higher education institutions. This study aims to determine accessibility needs of computer laboratories, libraries and websites for students with disabilities at Jordanian universities and colleges. The sample consists of staff in computer laboratories and libraries, web developers and e-learning staff to identify environmental and technological barriers from their perspective, as well as, to check their knowledge about assistive technology (AT) and issues related to accessibility. Questionnaires and expert review methods are used to test accessibility of websites in the investigated universities and colleges. Results indicated that current status of accessibility does not meet the expectations of equal access nor the needs of students with disabilities where there is shortages of ATs in computer labs and libraries, lack of awareness and insufficient training for the universities' staff and web developers related to accessibility issues, and all the evaluated universities web sites are inaccessible. Implications that should be contemplated by policy makers are highlighted and other recommendations are explored.