The purpose of this study was to evaluate physiological performance parameters of the elite karate-kumite athletes via the simulated karate performance test (SKPT). Sixteen elite male karate athletes from The Turkish National Team participated in the study- (age: 23.31±4.27 years, height: 173.4±4.91cm, weight: 68.8±8.17 kg, BMI: 22.85±1.96 kg/cm2 ). The SKPT protocol included 5 sets of 3 minutes simulated karate competition bouts, which included randomly timed 4 karate techniques (2 hands and 2 foot) in 7 seconds. With higher level of performance, the duration of the attacks increased whereas the rest periods shortened, but the total duration of the level remained constant (3 minutes). The blood lactate concentrations, heart rate, ventilation and oxygen consumption of the athletes were evaluated pre, mid and post SKPT. Statistically significant differences were found in all recorded parameters (p<0.05). During the simulated test peak oxygen consumption values were 41,69 ± 4,82 ml/kg/min at the 9th minute. Whereas heart rate, ventilation and oxygen consumption decreased to warm up values at the end of recovery, blood lactate values remained elevated during the whole recovery period. In conclusion, the simulation protocol implemented in this study has been found to produce physiological strain similar to real competition and can be used for performance evaluation of elite karate athletes.