This study focuses on the influence of gender, knowledge of, and attitude towards, disabilities on the stigmatization of persons with disabilities. The research design employed was a descriptive survey research design. The population of the study was civil servants in Ibadan, Oyo State, Nigeria. The sample was made up of 250 civil servants, using a multistage random sampling technique. Three instruments, the Knowledge of Disability Scale (KDS), Attitude Towards People with Disability Scale (ATPDS) and Stigmatization of People with Disability Scale (SPDS) were administered to the participants. Data collected were analyzed using the Pearson product-moment correlation and multiple regression analysis. The result revealed that there is a significant joint effect of gender, knowledge, and attitude on the stigmatization of people with disabilities in Oyo State, Nigeria. There was also relative contribution of all the independent variables, with knowledge and attitudes being the most important. The study recommended that civil servants should make an effort to improve their knowledge and attitude of disability as this will most likely reduce their level of stigmatization of persons with disabilities.