Objective: The objective of this study was to evaluate the participation rates and factors associated with nonparticipation among mothers living in low-income households who were invited to join a parent-education and -support research program delivered via the Internet with professional support. Methods: Four hundred and seventy-seven mothers of infants were contacted via a variety of recruitment methods, including presentations at clinics/classes, direct mailings, print advertisement, and Internet posts. Research staff attempted to contact these mothers by phone, to assess their eligibility, and interest in participation. For those who were eligible but declined participation, we assessed reasons for declining and collected demographic information. Results: Seventy-four percent of those eligible agreed to participate in the program. Statistical tests comparing participants and decliners on demographic variables found no significant differences on mother's age and marital status. There was a significant difference on baby's age due to many decliners in the prenatal period. Mothers were an average age of 28 years and most were married (65.6%). Our sample of low-income participants was diverse with a large number of Latina mothers. Approximately half had a high-school diploma or less but 84% reported being moderately or very comfortable using a computer and half had a computer at home.