This study investigated the influence of Pre-question and genre-based instructional strategies on science undergraduates' achievement in, and attitude to, reading. Using purposive sampling, two specialised universities in Nigeria were selected and stratified sampling was employed in assigning students to research groups based on gender and performance in a verbal ability test. Two hundred and eighty-five students participated in the study. Pre-post randomised block experimental design was used with three experimental groups and one control group. The experimental procedure involving Pre-question, genre-based instruction and a combination of Pre-question and genre-based instructional strategies were used for the experimental groups for four weeks whilst the control group received normal teacher input. Data were collected through a Reading Comprehension Achievement Test and Students' Attitude Questionnaire. Qualitative data, obtained from videotapes of classroom interactions, were subjected to conversation and interaction analyses and quantitative data were analysed with Analysis of Covariance (ANCOVA). The results indicate that although there was no significant main effect of instructional strategy on students' achievement in reading comprehension, there was significant main effect of instructional strategy on students' attitude to reading (F[subscript (3,231)] = 30.9; p < 0.05). Findings from the qualitative enquiry revealed that female students were more voluble and assertive in their responses probably because of the need to resist male domination whilst male students used discourse strategies to affirm their authority. The study indicated that the combination of pre-question and genre-based approach was the most effective in enhancing the students' attitude to reading. Reading is one of the most useful of the Language Arts skills which learners need for academic reasons and for lifelong learning. The globalised world demands that the second language student of science and technology demonstrates competence in reading materials in his specialist area in order to participate fully in his discourse community.