The aim of this study is to test the applicability of distance learning instrument education in undergraduate programs of higher education institutions. It is intended that a web-based experimental study has been designed for guitar education as a part of instrument education and that the designed study has been applied to the undergraduate students through distance learning. Guitar performance rating scale was used to collect data. Obtained data was analyzed by using Mann Whitney U and Wilcoxon nonparametric tests which measure and evaluate the significance of the difference between two mean scores. Following the analysis, the results were interpreted. According to the findings, it was concluded that the intended target behaviors were attained by the students who were taught by the conventional method and the students who were taught by the web-based distance education method at the same level and the web-based distance education has had the same effect on students' learning as the traditional instrument education practice. Suggestions in this context suggestions were also provided.