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ERIC Number: EJ1184703
Record Type: Journal
Publication Date: 2018-Jul
Pages: 5
Abstractor: As Provided
ISSN: ISSN-0021-9584
New Procedure to Readily Investigate Lactase Enzymatic Activity Using Fehling's Reagent
Leonello, Rocco; Savio, Matteo; Toaldo, Paola Baron; Bonomi, Renato
Journal of Chemical Education, v95 n7 p1238-1242 Jul 2018
Determination of enzymatic activity is a relevant experiment in teaching chemistry and biochemistry to high school and college students. A method for measuring the kinetic parameters of lactose cleavage by the enzyme lactase is described. This enzyme catalyzes the hydrolysis of lactose to galactose and glucose. The kinetic parameters can be determined easily and quickly by titration using Fehling's test for reducing sugars. In this way, it is possible to determine the rate of the reaction without using any instruments.
Division of Chemical Education, Inc and ACS Publications Division of the American Chemical Society. 1155 Sixteenth Street NW, Washington, DC 20036. Tel: 800-227-5558; Tel: 202-872-4600; e-mail:; Web site:
Publication Type: Journal Articles; Reports - Descriptive
Education Level: Higher Education
Audience: N/A
Language: English
Sponsor: N/A
Authoring Institution: N/A
Identifiers - Location: Italy
Grant or Contract Numbers: N/A