ERIC Number: EJ1180777
Record Type: Journal
Publication Date: 2018
Pages: 6
Abstractor: As Provided
ISBN: N/A
ISSN: ISSN-1470-8175
EISSN: N/A
A Laboratory Exercise for Detecting Microbial Contaminants
Shen, Jie; Zhu, Xiang; Jin, Kai; Li, Ya; Gu, YiTian; Huang, Jiahong; Qu, Yulan; Li, Xiaoyu
Biochemistry and Molecular Biology Education, v46 n3 p279-284 May-Jun 2018
The development and evaluation of a 6-hours laboratory class, based on capillary electrophoresis (CE) and the detection of microbial contaminants, is described. It can be easily scaled up or down, to suit class sizes up to 188 and completed in a shorter time scale. CE uses narrow-bore fused-silica capillaries to separate a complex array of large and small molecules. A laboratory exercise has been devised to illustrate how CE-based genetic analysis system processes DNA fragment analysis to detect three microbial contaminants. The protocol is relatively inexpensive and uses standard molecular biology reagents and equipment.
Descriptors: Science Laboratories, Science Instruction, Molecular Structure, Genetics, Molecular Biology, Program Development, Program Evaluation, Laboratory Experiments
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Publication Type: Journal Articles; Reports - Descriptive
Education Level: N/A
Audience: N/A
Language: English
Sponsor: N/A
Authoring Institution: N/A
Grant or Contract Numbers: N/A