The study aimed at identifying the degree of practicing academic freedom by faculty members in Jordanian public and private universities in the Northern Region from the point of view of the faculty members. The researcher applied the analytical descriptive survey method. The study population was composed of faculty members of Jordanian public and private universities in the Northern Region for the year 2017/2018. Their number was 1210 faculty members: 893 males and 308 females. The study sample was 445 males and 155 females, 50% of the study population. To achieve the objectives of the study, a study tool was developed. It is a questionnaire consisting of 50 items distributed on 5 domains. The findings of the study showed that the degree of practicing academic freedom by faculty members in Jordanian public and private universities in the Northern Region from the point of view of the faculty members on all domains of the tool was medium. Furthermore, there were no statistically significant differences at the level of significance (0.05 a =) in the degree of practicing academic freedom by faculty members in Jordanian universities due to the variable of the type of university, and the existence of differences in the degree of practicing academic freedom by faculty members in Jordanian public and private universities in the Northern Region can be attributed to the academic rank between those whose rank is professor and associate professor on the one hand, and the rank of assistant professor on the other, for the benefit of the rank of professor and associate professor, ie, in favor of the upper rank. The researcher recommended the need to increase the practice of academic freedom by the faculty members at the Jordanian public and private universities in the Northern Region.