The main objective of this study is to determine the training needs of prospective teachers for gender-aware media literacy training. In this study, within the scope of which qualitative research methods were employed, case study model was used. The study group was designated by using maximum variation sampling method, as one of the purposeful sampling methods. The study group of the research consisted of 32 prospective teachers in total (16 female students, 16 male students), receiving education in Gazi University Faculty of Education. Data was collected through a semi-structured interview form generated by the researchers and the form includes six headings and thirteen open-ended questions. In the process of analyzing data obtained as a result of the interviews performed face-to-face, content analysis method was used. In the light of the findings obtained from the research, it has become evident that prospective teachers are in need of training concerning the issues of accessing exact and reliable information, inquiring the reliability of information and sources, analyzing media messages, media editing, media literacy, gender equality and analyzing sexism in the media.