The aim of this study was to investigate the locomotor activity and anxiety changes in acute and chronic exposure to cigarette smoke in mice. In the chronic exposure group, mice were exposed to the smoke of 10 cigarettes for 5 days a week, over 5 consecutive weeks. In the acute group, the mice were exposed to the smoke of 10 cigarettes in one day. Locomotor activity tests and plus-maze tests were performed to determine the locomotor activity and anxiety levels of these mice. In the chronic exposure group, comparing with the both acute exposure and the control groups all parameters tested including distance traveled ( p<0.001), velocity of locomotor activity test (p<0.001), percentage of entrance through the open arm (p<0.001), and the duration of staying in the open arm of the plus-maze test (p<0.001) were significantly decreased .It has been concluded that the chronic cigarette smoke exposure resulted in decreased locomotor activity and increased anxiety levels in mice.