The purpose of this study is to determine the relationship between the entrepreneurship characteristics and metacognitive awareness levels of pre-service teachers. Qualitative research method is used in this study and the study is designed as a relational survey. The sample of the study was 441 pre-service teachers studying at the faculty of education of a public university in Turkey during the spring semester in 2015-2016 academic year. Random sampling was used to determine the sample group. The instruments used to collect the data in the study were "Metacognitive Awareness Scale for Pre-service Teachers (MASP)" developed by Duman (2013) and "Entrepreneurship Scale for University Students (ESUS)" developed by Yilmaz and Sünbül (2009). SPSS-22 package program was used to analyze the collected data. In determining the relationships between the scores from MASP and ESUS scales, pearson product-moment correlation analysis was conducted. In determining MASP's prediction of the scores from the ESUS scale multiple linear regression analysis was used. The results indicated a positive relationship at medium level between entrepreneurship characteristics and metacognitive awareness levels of pre-service teachers (r = 0.565 p<0.01). In addition, there are significant positive relationships between entrepreneurship characteristics and all sub-scales of MASP. It was found that the metacognitive awareness levels of pre-service teachers predicted their entrepreneurship characteristics at a rate of 43%. It is possible to say that activities towards improving metacognitive awareness could have positive impacts on entrepreneurship.