The purpose of this study is to compare the repeated sprint ability of amateur footbal players according to age and playing positions. For this purpose, 174 young amateur soccer players (age: 17.2 ± 1.8 years, height: 175.8 ± 7.5 cm, weight: 67.0 ± 9.8 kg) struggling in different playing positions participated voluntarily to the study. The players divided into six categories as goalkeepers (n: 16; age: 17.4 ± 1.4 years, height: 181.9 ± 6.1 cm, weight: 77.4 ± 9.8 kg), central defenders (n: 30; age: 16.9 ± 1.9 years, height: 179.3 ± 8.2 weight: 69.5 ± 11.2 kg), full-backs (n: 34; age: 17.3 ± 1.9 years, height: 174.9 ± 5.9 cm, weight: 66.6 ± 7.8 kg), central midfielders (n: 36; age: 17.9 ± 1,6 years; height: 174.3 ± 7.1 cm; weight: 67.1 ± 9.3 kg), wide midfielders (n: 30; age: 16.8 ± 1.6 years, height: 171.5 ± 5.1 cm, weight: 60.8 ± 7.4 kg) and forwards (n: 28; age: 16.6 ± 1.9 years, height: 175.9 ± 8.3 cm, weight: 65.6 ± 8.0 kg). In the study, a repeated sprint test was used to determine the repeated sprint ability of the football players, with an in field 34.2 meter long sprint run and a 25 second rest period after each run. In evaluating the data; descriptive statistics, one-way ANOVA and Kruskal-Wallis tests were used. According to the analysis results; the best test time (BTT), mean test time (MTT), and total test time (TTT) values in the repeated sprint test showed a statistically significant difference according to playing positions and age factor (p<0.05); On the other hand, fatigue index (FI) value showed a statistically significant difference according to playing positions (p<.05), but it didn't show any significant difference according to age (p>0.05). As a result, it can be argued that repeated sprint ability differs according to age and playing positions.