We have developed an OD3R method that can be applied on Biochemistry learning. This OD3R consists of 5 phases: orientation, decision, do, discuss, and reflect to connect lessons in the class with practice in the laboratory. Implementation of OD3R method was done in 2 universities in Yogyakarta to increase critical thinking skill and practical skill of the students. The critical thinking skills were assessed using modified Hoyo rubric according to student's ability in writing laboratory work report. Practical skill was assessed using a rubric with 6 practical skill categories: procedural skill, manipulative skill, observation skill, drawing skill, interpretation skill, and presentation skill. Implementation result shows that OD3R method can increase critical thinking skill and practical skill of the students. OD3R method is a learning method that is centered on the students and can be recommended to train thinking skill and practical skill of students in University.