It is important to note that social scientists have recently concentrated on the issue of values. People's thoughts, decisions, behaviors etc. values that have an important place in the explanations constitute the subject of this research. The main purpose of the research is to analyze whether the value orientations of the pre-service teacher' differ according to gender and branch variables. In the context with this research, the value orientations of the pre-service teachers attending the Pedagogical Formation Training Certificate Program which was held at Mardin Artuklu University in 2015 to 2016 academic year were revealed according to gender and branch variables. A mixed-methods approach incorporating both quantitative and qualitative methods was implemented in this research. The quantitative data were collected through the Portrait Values Questionnaire with the 40 questions developed by Schwartz and Bilsky, and the qualitative data were obtained by the means of semi-structured interview technique. A significant difference occurred in favor of the female participants with regard to gender variable. The pre-service teachers' value orientations also differed significantly taking into account their subject area variables.