Gender biases have often been observed in physical education (PE) classes, as many teachers adopt a male-biased perspective in teaching and learning. This might affect their evaluation of students' behavior and may lead students to accept and reproduce gender biases in other social contexts. The aim of this study was to examine whether PE teachers in Greece adopt gender biases in teaching and learning, favoring male students. The sample of the study comprised 392 elementary (n = 200) and secondary education students (n = 192) attending typical coeducational schools in Greece (M = 12.56, SD = 1.6). Participants completed the Perceived Physical Education Class Environment With Respect to the Achievement of Boys Questionnaire, which the researchers used to assess students' perceptions of gender inequalities during PE courses. The results revealed that male secondary education students reported higher scores of negative behaviors toward them, as compared to females, as well as compared to students in elementary school. The findings of this study provide valuable information on students' beliefs about gender inequalities in PE courses that may stimulate future research in this area.