The aim of this study was to develop a measurement instrument which is compatible with literature, of which validity and reliability are proved with the aim of determining interaction perceived by learners in online learning environments. Accordingly, literature review was made, and outline form of the scale was formed with item pool by taking 14 expert opinions. As a result of exploratory factor analysis of data obtained from participation of 177 learners from eight different universities, it was seen that the scale consisted of 30 items and 3 dimensions. These dimensions are learner-content interaction, learner-instructor interaction and learner-learner interaction. Cronbach Alpha reliability coefficient of the whole scale was 0.95. Confirmatory factor analysis showed that fit indexes of the structure provided a good and acceptable fit. Based on these findings, Perceptions of Online Interaction Scale is a valid and reliable tool that may be used in measuring interaction occurring in online learning environments.