The purpose of this study was to evaluate the effectiveness of psychological skills training (PST) in enhancing mental toughness among Turkish professional soccer team. Sixteen weeks of cognitive-behavioral conceptual framework-based PST program designed according to factors (confidence-constancy-control) of Sport Mental Toughness Questionnaire (SMTQ; Sheard et al., 2009) and (self-acceptance, positive relations with others, environmental mastery, purpose in life, personal growth and autonomy) scales of Psychological well-being (SPWB; Dierendonck, 2005). Twenty-six male soccer players between 18-33 years old (mean ± s: age 26.69 ± 3.54 years) voluntarily participated in this study. The relationship between mental toughness (MT) and psychological well-being was identified by conduction of Pearson Product Moment Correlations. To check effect of PST on MT and PWB in progress Paired sample t test was used. Throughout the intervention program, MT and PWB levels of participants significantly increased, in addition to self-acceptance, autonomy and positive relationships with others. Moreover, positive significant relationships were found between components of PWB and MT.