The main purpose of the present study is to examine the attitudes, perceptions and experiences of the teachers participating in the planning of teaching in the context of the Lesson Study. The present work, which is part of a wider research effort, followed a mixed methodological planning for reasons of triangulation. The survey was conducted from February 12 to April 1, 2016, in the second grade of the Secondary Education School in the prefecture of Attica and a total of 31 students, the Director as well as the four teachers of literature of the school unit participated in it. The results of the survey show that the participating teachers were positively influenced by the detailed planning and preparation of the research lesson, gaining useful experience and valuable knowledge during the implementation of the Lesson Study phases. In addition to that, their attitudes and perceptions about the meetings and workshops carried out within Lesson Study were positively evaluated.