NotesFAQContact Us
Search Tips
Peer reviewed Peer reviewed
Direct linkDirect link
ERIC Number: EJ1166977
Record Type: Journal
Publication Date: 2018-Jan
Pages: 5
Abstractor: As Provided
ISSN: ISSN-0021-9584
UV-Vis Spectrophotometric Analysis and Quantification of Glyphosate for an Interdisciplinary Undergraduate Laboratory
Felton, Daniel E.; Ederer, Martina; Steffens, Timothy; Hartzell, Patricia L.; Waynant, Kristopher V.
Journal of Chemical Education, v95 n1 p136-140 Jan 2018
Glyphosate (N-(phosphonomethyl)glycine) is the most widely used herbicide on earth. A simple assay to quantify glyphosate concentrations in environmental samples was developed as part of an interdisciplinary effort linking introductory laboratory courses in chemistry, biology, and microbiology. In this 3 h laboratory experiment, students used UV-vis spectroscopy to quantify glyphosate in prepared unknowns and supernatants from glyphosate-treated soil samples. Regression analysis indicated that the assay is linear up to 20.0 ppm, making it particularly useful for detection of low levels of glyphosate in environmental samples. The assay can be used to quantify the activity of glyphosate-degrading soil microorganisms by comparing glyphosate levels between autoclaved and nonautoclaved soil slurries.
Division of Chemical Education, Inc and ACS Publications Division of the American Chemical Society. 1155 Sixteenth Street NW, Washington, DC 20036. Tel: 800-227-5558; Tel: 202-872-4600; e-mail:; Web site:
Publication Type: Journal Articles; Reports - Descriptive
Education Level: Higher Education
Audience: N/A
Language: English
Sponsor: N/A
Authoring Institution: N/A
Grant or Contract Numbers: N/A