During 2010-2012, three professional development workshops brought together K-12 educators and scientists conducting research in the geographic and ecological context of Alaska's three large marine ecosystems (Bering Sea/Aleutians, Gulf of Alaska, and Arctic Ocean). Educators successfully applied new scientific knowledge gained from their interactions with scientists through the collaborative development of lesson plans that were place-based and culturally responsive to Alaska Native cultures. Over the course of the three workshops, we refined a model for incorporating cultural responsiveness into workshop design, employed an innovative systemic traditional knowledge framework, and developed a rubric to evaluate the lesson plans in terms of cultural responsiveness. Key factors that increased the impact of a single professional development workshop on the ability of the K-12 educators to produce culturally responsive lesson plans included (1) participation of experienced teachers as mentors, (2) opportunities for workshop participants to interact with community members and culture bearers, and (3) embedding the training within a longer-term program of curriculum development and professional development in a school district for which cultural responsiveness was a high priority.