Listening comprehension is a complex skill particulaly in mastered by non-native speaker settings. This research aimed at finding out the effect of multimedia application on students' listening. The research design is experimental, with a t-test. The population is the sixth semester of HKBP Nommensen University at the academic year of 2016/2017, six classes of listening comprehension, 3 classes for experiments and the other for control, each class of 20 students. The findings states that the Tcount = 14.68 > Ttable = 2.02 in a significance 0.05 and degree of freedom 38. It means that, multimedia application is effective than conventional media. Multimedia in this study can be used by other lecturer or teacher as the optional instrument in teaching listening comprehension.