ERIC Number: EJ1159474
Record Type: Journal
Publication Date: 2017-Oct
Pages: 6
Abstractor: As Provided
ISBN: N/A
ISSN: ISSN-0021-9584
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Available Date: N/A
Demonstrating Basic Properties of Spectroscopy Using a Self-Constructed Combined Fluorimeter and UV-Photometer
Kvittingen, Eivind V.; Kvittingen, Lise; Melø, Thor Bernt; Sjursnes, Birte Johanne; Verley, Richard
Journal of Chemical Education, v94 n10 p1486-1491 Oct 2017
This article describes a combined UV-photometer and fluorimeter constructed from 3 LEDs and a few wires, all held in place with Lego bricks. The instrument has a flexible design. In its simplest version, two UV-LEDs (355 nm) are used as light source and to detect absorption, and a third LED, in the visible spectrum (e.g., 525 nm), is used to detect fluorescence. Various experiments are described: fluorescence as a function of concentration and light source intensity; quantitative measurements of quinine in tonic water, using absorption and fluorescence measurements, with results in line with those obtained on scientific instruments; a demonstration of the inner filter effect reducing fluorescence at higher concentrations; investigation of the effect of drinking tonic water on the fluorescence of quinine in urine; temperature effects on fluorescence; chloride-ion quenching of fluorescence with result comparable to those previously reported; and, finally, a demonstration of phosphorescence.
Descriptors: Spectroscopy, Scientific Principles, Scientific Methodology, Light, Science Equipment, Science Experiments, Measurement Equipment, Measurement Techniques, Secondary School Science, College Science
Division of Chemical Education, Inc and ACS Publications Division of the American Chemical Society. 1155 Sixteenth Street NW, Washington, DC 20036. Tel: 800-227-5558; Tel: 202-872-4600; e-mail: eic@jce.acs.org; Web site: http://pubs.acs.org/jchemeduc
Publication Type: Journal Articles; Reports - Descriptive
Education Level: Secondary Education
Audience: N/A
Language: English
Sponsor: N/A
Authoring Institution: N/A
Grant or Contract Numbers: N/A
Author Affiliations: N/A