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ERIC Number: EJ1159474
Record Type: Journal
Publication Date: 2017-Oct
Pages: 6
Abstractor: As Provided
ISSN: ISSN-0021-9584
Demonstrating Basic Properties of Spectroscopy Using a Self-Constructed Combined Fluorimeter and UV-Photometer
Kvittingen, Eivind V.; Kvittingen, Lise; Melø, Thor Bernt; Sjursnes, Birte Johanne; Verley, Richard
Journal of Chemical Education, v94 n10 p1486-1491 Oct 2017
This article describes a combined UV-photometer and fluorimeter constructed from 3 LEDs and a few wires, all held in place with Lego bricks. The instrument has a flexible design. In its simplest version, two UV-LEDs (355 nm) are used as light source and to detect absorption, and a third LED, in the visible spectrum (e.g., 525 nm), is used to detect fluorescence. Various experiments are described: fluorescence as a function of concentration and light source intensity; quantitative measurements of quinine in tonic water, using absorption and fluorescence measurements, with results in line with those obtained on scientific instruments; a demonstration of the inner filter effect reducing fluorescence at higher concentrations; investigation of the effect of drinking tonic water on the fluorescence of quinine in urine; temperature effects on fluorescence; chloride-ion quenching of fluorescence with result comparable to those previously reported; and, finally, a demonstration of phosphorescence.
Division of Chemical Education, Inc and ACS Publications Division of the American Chemical Society. 1155 Sixteenth Street NW, Washington, DC 20036. Tel: 800-227-5558; Tel: 202-872-4600; e-mail:; Web site:
Publication Type: Journal Articles; Reports - Descriptive
Education Level: Secondary Education
Audience: N/A
Language: English
Sponsor: N/A
Authoring Institution: N/A
Grant or Contract Numbers: N/A