Due to its well documented benefits, it has been recommended to integrate mindfulness into health promotion programming. The purpose of this study was to determine if mindfulness was impacted by demographic variables and/or the completion of a stress management unit including mindfulness and application activities focused on paying attention and living in the present. Students completed (n = 225) the 15-item Mindful Attention Awareness Scale (MAAS) at the beginning and end of a 15-week semester. Student completion of a stress management unit including mindfulness and application activities focused on paying attention and living in the present did not result in improved mindfulness. The extent that instructors covered mindfulness in the lab portion of the course, as well as potentially higher stress levels at the end of the semester, may be factors associated with lower post-course mindfulness. These results, combined with previous research showing mindfulness-based programs to be effective in stress reduction, support the need to directly educate students about mindfulness. It may be advantageous to increase the focus on mindfulness in university lifetime wellness curricula, in addition to improving instructor training and monitoring course fidelity among large multiple-section courses.