The study aims to investigate the effect of "Physical Education and Sport Culture" (PESC) course on the attitudes of preservice classroom teachers towards physical education and sports. The one group pre-test post-test design among experimental models which is included in quantitative research designs was employed in the study. The study was conducted on a total of 90 sophomores (66 female and 24 male) taking PESC course offered in Erzincan University Faculty of Education Classroom Teaching Program during the fall term in 2016-2017 academic year. The "Physical Education and Sports Scale" was used to measure attitudes of students towards physical education and sports both at the beginning and end of the term, and the "personal details form" prepared by the researcher was used for data collection purpose. An analysis of pre-test post-test attitude scores of preservice teachers taking PESC course indicated a significant difference between pre-course (??¯=94.43) and post-course attitude scores (??¯=97.49) (t [subscript (89)] =-2.291, p<0.05). The size of effect that is calculated after the test (d=0.25) showed a medium level of difference. The results concluded that PESC course taken by preservice classroom teachers has a positive effect on their attitudes towards physical education and sports.