ERIC Number: EJ1152932
Record Type: Journal
Publication Date: 2017-Sep
Abstractor: As Provided
Novelty and Fear Conditioning Induced Gene Expression in High and Low States of Anxiety
Donley, Melanie P.; Rosen, Jeffrey B.
Learning & Memory, v24 n9 p449-461 Sep 2017
Emotional states influence how stimuli are interpreted. High anxiety states in humans lead to more negative, threatening interpretations of novel information, typically accompanied by activation of the amygdala. We developed a handling protocol that induces long-lasting high and low anxiety-like states in rats to explore the role of state anxiety on brain activation during exposure to a novel environment and fear conditioning. In situ hybridization of the inducible transcription factor "Egr-1" found increased gene expression in the lateral nucleus of the amygdala (LA) following exposure to a novel environment and contextual fear conditioning in high anxiety-like rats. In contrast, low state anxiety-like rats did not generate "Egr-1" increases in LA when placed in a novel chamber. "Egr-1" expression was also examined in the dorsal hippocampus and prefrontal cortex. In CA1 of the hippocampus and medial prefrontal cortex (mPFC), "Egr-1" expression increased in response to novel context exposure and fear conditioning, independent of state anxiety level. Furthermore, in mPFC, "Egr-1" in low anxiety-like rats was increased more with fear conditioning than novel exposure. The current series of experiments show that brain areas involved in fear and anxiety-like states do not respond uniformly to novelty during high and low states of anxiety.
Descriptors: Novelty (Stimulus Dimension), Fear, Conditioning, Genetics, Anxiety, Brain, Brain Hemisphere Functions, Experiments, Responses
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Publication Type: Journal Articles; Reports - Research
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