This study was conducted to determine the acute changes after the 20 m shuttle run test in the biochemical parameters and heart rates of wrestlers competing in international competitions on a study population of 21 wrestlers. The mean age of participants was 24.14±2.01 years, mean heights 176±0.06 cm, body weight 76.48±14.76 kg, MaxVO[subscript 2] 52.78±3.20 ml/kg/min, and mean BMI 24.44±2.63 kg/m[superscript 2]. 20-meter run and sit-up test was applied to measure the MaxVO[subscript 2] scores of included subjects. Heart rates were monitored using a Polar heart rate monitor before and after the tests. The blood samples that collected at the same time intervals from the vena cephalica were stored in tubes without anticoagulants. The samples were then centrifuged for 10 minutes at 5000 rpm to obtain serum samples, and analyzed. The data obtained were treated using SPSS 15.0 software. This study showed that there was a significant increase (p<0.05) in post-test glucose, HDL-cholesterol and urea concentrations compared to pre-test levels. While the post-test LDL cholesterol concentration was significantly reduced (p<0.05), acute changes in other biochemical parameters were found to be statistically insignificant (p>0.05). It can be concluded that mean glucose, HDL and urea were increased while LDL was reduced after the 20 m shuttle run test in wrestlers.