Background: Weight gain and lifestyle behaviors during college may contribute to future health problems. This population may not have sufficient self-monitoring skills to maintain healthy lifestyle behaviors. Purpose: The purpose of this study was to determine the relationship between usages of mobile health applications (apps) designed to track diet and physical activity and health-related behaviors of college students. Methods: In a cross-sectional study, 401 university students completed a survey to assess eating behavior, physical activity, and health-related lifestyle choices and mobile health app usage. Results: Mobile health app users had significantly higher scores for eating behavior than nonusers, and the impact of using more than one type of mobile health app significantly improved eating behavior. Most participants also identified app use with feeling healthier, better self-monitoring of food intake and exercise, and having more motivation to eat healthier and increase physical activity. Discussion: Use of mobile health apps may have a positive effect on eating behavior, and demographic background appears to be influential with regard to health-related behaviors. Translation to Health Education Practice: Health Educators need to consider the use of apps as a supplementary component of a health promotion program to assist individuals who want to make improvements in their overall health and to prevent chronic disease.