The aim of this study was to evaluate the efficacy of yoga on spiritual intelligence in air traffic controllers in Tehran flight control center. This was a quasi-experimental research and the study population includes all air traffic controllers in Tehran flight control center. The sample consisted of 40 people of the study population that were selected through convenience sampling method and were randomly divided into experimental group (20) and control group (n = 20). This research was conducted through training yoga on the air traffic controllers to experimental group compared with the control group. The data were evaluated as the changes resulted after the intervention (post-test) compared to the previous condition (pre-test). King's (2008) Spiritual Intelligence Self-Report Inventory (SISRI) was used to measure the variables. The data were analyzed by analysis of covariance. The results showed that yoga exercises had a significant effect on spiritual intelligence and its components (critical thinking, personal meaning production, expanded state of consciousness, transcendental consciousness) in the subjects.