Objective: This study sought to develop and evaluate the psychometric properties of a tool designed to assess social work students' knowledge of and perceptions and attitudes toward human trafficking. To achieve this aim, the Perceptions, Knowledge, and Attitudes toward Human Trafficking Questionnaire (PKA-HTQ) was developed and its psychometric properties were evaluated. Specifically, the factor structure and the internal consistency of the PKA-HTQ were evaluated. Methods: Exploratory factor analysis (EFA) and a replication EFA were conducted on two independent samples of university students, an initial validation (n ¼ 325), and cross-validation (n = 212) sample. Findings: The EFA revealed a three-factor structure, that is, self-appraisal of knowledge/skills (a = 0.89), worldview (a = 0.78), and help-seeking behavior (a = 0.66); this three-factor structure was supported by replication EFA. Conclusion: The PKA-HTQ questionnaire shows promise as a meaningful, potentially reliable and valid measure.