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ERIC Number: EJ1151305
Record Type: Journal
Publication Date: 2017-Aug
Pages: 7
Abstractor: As Provided
ISSN: ISSN-0021-9584
Site-Directed Mutagenesis Study of an Antibiotic-Sensing Noncoding RNA Integrated into a One-Semester Project-Based Biochemistry Lab Course
Gerczei, Timea
Journal of Chemical Education, v94 n8 p1083-1089 Aug 2017
A laboratory sequence is described that is suitable for upper-level biochemistry or molecular biology laboratories that combines project-based and traditional laboratory experiments. In the project-based sequence, the individual laboratory experiments are thematically linked and aim to show how a bacterial antibiotic sensing noncoding RNA (the ykkCD riboswitch) recognizes its target antibiotic, tetracycline. The novelty of the curriculum lies in its extensive coverage of bioinformatics as well as its focus on synthesis, purification, and functional studies of a biologically active RNA. Further advantage of the curriculum is that the model system, the ykkCD riboswitch, regulates expression of a gene that is involved in bacterial antibiotic resistance. Hence, the project makes this important issue part of the undergraduate biochemistry curriculum. The curriculum is available in Open Access format as part of a biochemistry laboratory manual by Gerczei and Pattison. This manuscript summarizes the curriculum and provides suggestions to the instructor regarding its implementation.
Division of Chemical Education, Inc and ACS Publications Division of the American Chemical Society. 1155 Sixteenth Street NW, Washington, DC 20036. Tel: 800-227-5558; Tel: 202-872-4600; e-mail:; Web site:
Publication Type: Journal Articles; Reports - Descriptive
Education Level: Higher Education
Audience: Teachers
Language: English
Sponsor: N/A
Authoring Institution: N/A
Identifiers - Location: Indiana
Grant or Contract Numbers: N/A