Staff turnover especially among lecturers in private universities is high due to poor and disappointing conditions of service. This negatively affects effective teaching, learning and research. The purpose of the study was to examine the causes of staff turnover in private universities, using the Perez University College in Winneba as a case study. The study employed the descriptive survey design to collect data from forty (40) lecturers and twenty (20) administrative staff. The convenience sampling technique was therefore used to obtain the sample size of 60 respondents. Questionnaire and interview guides were used to collect data. The study revealed that lecturers and top administrators were poorly paid. There was low research output among lecturers in private universities. Libraries lack current publications and ICT connectivity to support teaching, learning and research. The study recommended the improvement in the conditions of service of staff. It also recommended the establishment of a research fund to motivate lecturers to increase research output as well as ICT connectivity to support effective teaching, learning and research in private universities in Ghana.