Technology provides new methods and approaches for educational activities. Therefore, teachers should improve their ability and knowledge to integrate technology into instruction. The use of technology-based learning environment which is effectively used to improve the technological pedagogical content knowledge of pre-service teachers has a crucial importance for the training of pre-service teachers. In this regard, the purpose of this study is to investigate the technological pedagogical content knowledge (TPACK), TPACK related self-confidence, and perception of pre-service middle school mathematics teachers in terms of instructional technologies. In this study, TPACK Survey, TPACK Self-Confidence Survey, and TPACK Perception Survey were administered to 427 pre-service middle school mathematics teachers in elementary mathematics education program. The data were analyzed quantitatively. The data analysis revealed that there was a significant relationship between gender and perception towards technology. Moreover, it might be concluded that pre-service teachers improve their knowledge and self confidence to use technology in elementary mathematics education programs. Lastly, considering the finding that there was a relationship between the use of technology and self-confidence towards the use of technology, it might be inferred that self-confidence of pre-service teachers towards the use of educational technologies increases with the use instructional tools.