The aim of this study was to compare the values of heart rate (HR) and blood lactate (BL) of kickboxers during kickboxing match. This study was conducted with the participation of 18 male and 16 female sportsman in Turkish Kickboxing championship, an organization of Turkish Kickboxing Federation. After the participant athletes read and signed informed voluntary consent form (BGOF) prepared for them, the measurements were collected. While no significant difference was found between ages, pre-match HR, post-match HR, pre and post-match BL of male and female Kickboxers, a significant difference was statistically found between the height (P?0.05) and body weights (P?0.01) of athletes. Age distribution of athletes was determined as 14-23 and mean age distribution was determined as 17.03 ± 2.41. HR pressures of athletes differs according to pre-match and post-match periods (P?0.001). As HR frequencies increase by 29.4% according to pre-match HR regardless of the gender, this increase was determined as 31.4% for male athletes and 27.2% for female athletes. While post-match BLs of athletes increase by 4.02 times compared to pre-match regardless of the gender, this increase was determined as 4.20 for male athletes and 3.82 for female athletes. Kickboxing is a combat sport which has high intensity of anaerobic physical fitness. Matches and rounds for HR and BL concentrations show the importance of the aerobic metabolism in kickboxing. However, the significant rise of HR and BL during simulated match indicates that the anaerobic metabolism is also important in kickboxing.